【英文标准名称】:Foodstuffs-Methodsofanalysisforthedetectionofgeneticallymodifiedorganismsandderivedproducts-Qualitativenucleicacidbasedmethods
【原文标准名称】:食品.基因改良有机物和衍生产品的检测用分析方法.基于定性的核酸方法
【标准号】:ISO21569-2005
【标准状态】:现行
【国别】:国际
【发布日期】:2005-06
【实施或试行日期】:
【发布单位】:国际标准化组织(IX-ISO)
【起草单位】:ISO/TC34
【标准类型】:()
【标准水平】:()
【中文主题词】:规范(验收);试验;定义;食品;微生物;食品检验;分析方法;试验报告;验证;说明书;在实验室条件下;分析;质量保证;化学分析和试验;性能要求;PCR;筛选法;DNA;聚合酶链式反应;链式反应;实验室;微生物学;基因产品;基因改良;污染;实验室设备;实验室试验;微生物分析;生物体;最低要求;聚合酶;微生物食品;核酸;遗传工程
【英文主题词】:Analysis;Chainreactions;Chemicalanalysisandtesting;Contamination;Definitions;DNA;Foodinspection;Foodproducts;Foodtesting;Genemodified;Geneproduct;Geneticengineering;Invitro;Instructions;Investigations;Laboratories;Laboratoryequipment;Laboratorytesting;Laboratorytests;Methodsofanalysis;Microbialfoods;Microbiologicalanalysis;Microbiology;Microorganisms;Minimumrequirements;Nucleicacids;Organisms;PCR;Performancerequirements;Polymerase;Polymerasechainreaction;Qualityassurance;Screening-method;Specification(approval);Testing;Verification
【摘要】:ThisInternationalStandarddescribestheproceduretoqualitativelydetectgeneticallymodifiedorganisms(GMOs)andderivedproductsbyanalysingthenucleicacidsextractedfromthesampleunderstudy.Themainfocusisonpolymerasechainreaction(PCR)basedamplificationmethods.Itgivesgeneralrequirementsforthespecificdetectionandidentificationoftargetnucleicacidsequences(DNA)andfortheconfirmationoftheidentityoftheamplifiedDNAsequence.Guidelines,minimumrequirementsandperformancecriterialaiddowninthisInternationalStandardareintendedtoensurethatcomparable,accurateandreproducibleresultsareobtainedindifferentlaboratories.ThisInternationalStandardhasbeenestablishedforfoodmatrices,butcouldalsobeappliedtoothermatrices(e.g.feedandplantsamplesfromtheenvironment).SpecificexamplesofmethodsareprovidedinAnnexesAtoD.
【中国标准分类号】:X04
【国际标准分类号】:67_050
【页数】:69P;A4
【正文语种】:英语